In the comics, in order for Nico's magic to work, Nico must make a blood sacrifice by making herself bleed by any means necessary (mostly by cutting herself). But in the show, it was change to having Nico pricking her thumb to a needle in the staff, reading her DNA in the process. This was done to avoid controversy about self-harm, which seen as a response to another teen drama, 13 Reasons Why.
In the comics, Molly is a mutant who eventually makes ties with the X-Men. In the series, the nature of her powers are left ambiguous because the film rights X-Men and mutants are owned by 21st Century Fox. However, on December 14th, 2017 while the first season was airing, Disney announced their intentions to purchase Fox's film rights which include the X-Men and Fantastic Four.
The code word that the Pride uses as an Amber Alert, 'Tsunami', and also the title of episode 8, is a reference to the Marvel Comics imprint of the same name. Tsunami was a (now defunct) line of manga-influenced comics Marvel debuted in 2003, of which Runaways was the most popular.
The Runaways was originally going to be a film in Phase One, with production began on May 2008. Brian K. Vaughn was hired to write the screenplay, Peter Sollett meant to direct the film, and Drew Pearce to write a new script. However, the project was shelved in 2013 due to the success of The Avengers, with Phase Three being the earliest time for The Runaways to become a film but it was left unannounced. The creators of Gossip Girl, Josh Schwartz and Stephanie Salvage, later made a deal with Marvel Television to turn the almost-abandoned project in to a TV series.
The trivia items below may give away important plot points.
In the comics, original team consisted of Nico Minoru (daughter of witches), Karolina Dean (daughter of alien invaders), Molly Hayes (daughter of telepathic mutants), Gertrude Yorkes (daughter of time-travelling thieves), Chase Stein (son of mad scientist) and Alex Wilder (son of mob bosses). They are later joined by Victor Mancha (a cyborg whose father is revealed to be Ultron), Xavin (a shape shifting super-skrull in training) and Klara Prast (a mutant child bride from the 20th Century with the ability to manipulate and communicate with plants).
In episode 6 when the kids are riding in the limo, the driver is Stan Lee, Editor Emeritus of Marvel Comics who, along with Jack Kirby, created many of Marvel's initial roster of heroes. Stan has cameos in most of Marvel's movies.
In the show, Alex gave Gert a poster for a movie theater that plays old films, with one of those films being 'Arsenic and Old Lace'. In the comics, Gert used 'Arsenic' as a codename and gave her pet dinosaur the name 'Old Lace'. This bit in the show and comics both references the movie and play 'Arsenic and Old Lace', which tells the dark humorous story of a man finding out his family has a history of insanity.
Contrary to popular belief, Karolina Dean is NOT the first LGBT character in the Marvel Cinematic Universe. Jeri Hogarth from Jessica Jones has that distinction. However, Karolina is the first major superhero in the MCU to be a member of the LGBT community.